Spike2 version 2 for Windows/Macintosh

Spike2 laboratory software delivers powerful data capture and analysis, stimulus sequencing and experimental control using a CED Power1401, micro1401 or 1401plus data acquisition peripheral for PC or Macintosh host computers. Specifically developed for life science research, Spike2 is already used in hundreds of laboratories world-wide for a diverse range of applications.

Typical applications

Extracellular physiology Teaching Sleep Studies

Vibration monitoring Clinical neurophysiology Gastro-intestinal research

Electromyography Force measurement Cardiovascular research

Behavioural studies Evoked response Signal conditioner control

Data acquisition

Record data to hard disk continuously or at timed or externally triggered intervals Micro1401 with Spike2 expansion
CED micro1401 with the Spike2 expansion unit

Sample up to 16 waveform inputs at independent frequencies up to 80kHz

Log digital (TTL compatible) events, such as the output from a waveform discriminator, or a synchronising pulse, on up to 8 channels to a resolution of 2 microseconds

Flag data files with text or digital markers to indicate external events and facilitate selective review and analysis

Classify multi-unit spike activity into individual units on multiple channels (8 on PC and Power PC Mac, 1 on the 68k Mac)

Program waveform and digital output to generate stimulus sequences and to control experimental conditions

Configure dynamically programmable amplifiers (CED 1902 / Axon CyberAmp) and with the PC and Power PC Mac, the waveform discriminator CED 1401-18

Data display and analysis

Mouse-driven display options include:
Menu driven signal capture and analysis

Multiple windows with independent time bases

X and Y axis pan and zoom

Data review during acquisition

Display of event data as mean or instantaneous, frequency, raster or rate histogram

Mouse-driven analysis functions are easy to perform during data acquisition or in off-line review.

Event analyses Waveform analyses

Event total count and mean frequency Signal mean, area and gradient

Event interval or phase histogram Triggered average

Event correlations Waveform correlation

Post stimulus and peri-stimulus histogram Spectral analysis

Waveform discrimination

The CED script language

A macro language is available for automating procedures and for developing custom analyses. This script language provides a genuinely versatile analysis environment both during data capture and in off-line review. Whilst many users write their own scripts, CED continues to develop and maintain a library of scripts which are available to customers and provide a wide range of tailor-made functions. CED script language features:

Specially developed high-level commands for performing complex analyses with a single instruction

Programmable toolbars, pop-up windows and dialog boxes for developing a custom user-interface

Familiar control structures (for - next loops, procedures, etc.)

Comprehensive debugging tools - breakpoints, single-stepping and variable values

Automated signal analysis
Custom user-interface

System requirements

Spike2 for Macintosh requires a CED 1401plus or CED micro1401 intelligent laboratory interface and a Macintosh with PCI and operating system 7.0 or later. We recommend that the Macintosh has at least 8MB RAM fitted.

Spike2 for Windows requires a CED Power1401, 1401plus or CED micro1401 intelligent laboratory interface and an IBM compatible PC with Windows 3.1, 95, 2000 or NT. We recommend at least 8MB RAM is fitted.

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Choose between Signal and Spike2

Extracellular physiology	Teaching	Sleep Studies
Vibration monitoring	Clinical neurophysiology	Gastro-intestinal research
Electromyography	Force measurement	Cardiovascular research
Behavioural studies	Evoked response	Signal conditioner control

	Record data to hard disk continuously or at timed or externally triggered intervals	CED micro1401 with the Spike2 expansion unit
	Sample up to 16 waveform inputs at independent frequencies up to 80kHz
	Log digital (TTL compatible) events, such as the output from a waveform discriminator, or a synchronising pulse, on up to 8 channels to a resolution of 2 microseconds
	Flag data files with text or digital markers to indicate external events and facilitate selective review and analysis
	Classify multi-unit spike activity into individual units on multiple channels (8 on PC and Power PC Mac, 1 on the 68k Mac)
	Program waveform and digital output to generate stimulus sequences and to control experimental conditions
	Configure dynamically programmable amplifiers (CED 1902 / Axon CyberAmp) and with the PC and Power PC Mac, the waveform discriminator CED 1401-18

Mouse-driven display options include:		Menu driven signal capture and analysis
	Multiple windows with independent time bases
	X and Y axis pan and zoom
	Data review during acquisition
	Display of event data as mean or instantaneous, frequency, raster or rate histogram

Mouse-driven analysis functions are easy to perform during data acquisition or in off-line review.

Event analyses		Waveform analyses
	Event total count and mean frequency		Signal mean, area and gradient
	Event interval or phase histogram		Triggered average
	Event correlations		Waveform correlation
	Post stimulus and peri-stimulus histogram		Spectral analysis
			Waveform discrimination

	Specially developed high-level commands for performing complex analyses with a single instruction
	Programmable toolbars, pop-up windows and dialog boxes for developing a custom user-interface
	Familiar control structures (for - next loops, procedures, etc.)
	Comprehensive debugging tools - breakpoints, single-stepping and variable values